effect of human sperm chromatin anomalies on fertilization outcome post icsi

introduction: the aim of this study was to evaluate the effect of sperm chromatin anomalies on fertilization outcome post icsi. material and methods: therefore along with semen parameters, cma3 staining for protamine deficiency, aniline blue staining for excessive histones, sds for sperm chromatin stability and sds+edta for ability of sperm to undergo decondensation were carried out on 55 semen samples from patients referring for icsi to isfahan fertility and infertility cente. result: the results showed that among the aforementioned tests and semen parameters, only cma3 showed a significant correlation with fertilization outcome post icsi. patients were also grouped according to cma3 level of <30% or >30 % or fertilization rate of <50%, or >50%. the results showed that the mean percentage fertilization and mean percentage of cma3 positivity is different inboth groups respectively. the area under roc curve showed that cma3 is a highly sensitive and specific test for prediction of fertilization outcome post icsi. conclusion: therefore, it can be concluded that sperm protamine deficiency has profound effect on fertilization failure in icsi.